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recombinant human cxcl1 gro alpha protein  (R&D Systems)


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    Structured Review

    R&D Systems recombinant human cxcl1 gro alpha protein
    Recombinant Human Cxcl1 Gro Alpha Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+gro/Recombinant+Human+CXCL1%2FGRO+alpha+Protein/pm41833106-49-0-21
    Average 94 stars, based on 27 article reviews
    recombinant human cxcl1 gro alpha protein - by Bioz Stars, 2026-09
    94/100 stars

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    Comparison:


    Recombinant:


    Article Title: Sulfotyrosines of the Kaposi's Sarcoma-Associated Herpesvirus G Protein-Coupled Receptor Promote Tumorigenesis through Autocrine Activation
    Article Snippet: .. Recombinant human GRO- and IP-10 were purchased from R&D Systems, Inc.; [125I]GRO- and [125I]IP-10 were purchased from PerkinElmer. .. The tyrosine-sulfated peptide DDDESWNETLNMSGY26DY28SGNFS and the unsulfated equivalent, which correspond to the primary sequence of residues 12 through 33 of vGPCR, were synthesized to 95% purity by Bio-synthesis, Inc. (sulfated tyrosines are numbered and in boldface).

    Article Title: Smooth and rough lipopolysaccharide phenotypes of Brucella induce different intracellular trafficking and cytokine/chemokine release in human monocytes.
    Article Snippet: An allophycocyanin-conjugated mAb to CD14 was purchased from Beckman Coulter; mAb to GRO- (CXCL1) from Sigma-Aldrich; mAb to MCP-1 (CCL1), IL-6, IL-10 and TNF- from BD PharMingen; mAb to IL-8 (CXCL8), MIP-1 (CCL3), MIP-1 (CCL4) and RANTES (CCL5), as well as a biotinylated secondary Ab from R&D Systems Biotech; and a streptavidin-peroxidase conjugate from Boehringer Mannheim (now Roche Diagnostics). .. Recombinant human GRO- , MCP-1, IL-10, IL-8, MIP-1 , MIP-1 , and RANTES were all obtained from R&D Systems Biotech; TNF- from BD PharMingen; and IL-6 from PBH Pharma Biotechnologie, Hannover, Germany. ..

    Article Title: Lysophosphatidic acid up-regulates expression of growth-regulated oncogene-alpha, interleukin-8, and monocyte chemoattractant protein-1 in human first-trimester trophoblasts: possible roles in angiogenesis and immune regulation.
    Article Snippet: .. Recombinant human GRO- , IL-8, and MCP-1 neutralizing antibodies were obtained from R&D Systems (Minneapolis, MN). .. Antibodies to human phospho-protein kinaseC(PKC),p38,ERK,Akt,andc-JunN-terminalkinase (JNK) were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA).

    Article Title: Molecular expression of the alpha-chemokine rabbit GRO in Escherichia coli and characterization of its production by lung cells in vitro and in vivo.
    Article Snippet: Polyclonal goat IgG was purified from serum using protein G columns (Pierce), and a portion was biotinylated (Pierce). .. The specificity of the goat antisera was tested by Western blotting using biotinylated goat anti-RabGRO IgG or biotinylated nonimmune IgG and recombinant rabbit IL-8, rabbit monocyte chemotactic protein-1 (MCP-1) (18), recombinant human GRO-a (R & D Systems, Inc., Minneapolis, MN), and rRabGRO protein. .. Radioimmunoprecipitation of Rabbit GRO Protein from Stimulated AM—Rabbit AM (2 3 106 cells/ml) were incubated for 1 h at 37 °C in 24-well plates containing 1 ml of labeling medium containing 250 mCi of [35S]Cys (DuPont NEN).

    Article Title: Chemotactic activity of CXC chemokines interleukin-8, growth-related oncogene-alpha, and epithelial cell-derived neutrophil-activating protein-78 in urine of patients with urosepsis.
    Article Snippet: .. For determination of GRO-a levels, purified monoclonal mouse anti–human GRO-a (4 mg/mL; R&D Systems, Abingdon, UK) was used as a coating antibody, biotinylated affinity-purified goat IgG anti–human GRO-a (20 ng/mL; R&D Systems) was used as a detecting antibody, and recombinant human GRO-a (R&D Systems) was used as the standard. .. For determination of ENA-78, monoclonal mouse anti–human ENA-78 (1 mg/mL; R&D Systems) was used as a coating antibody, biotinylated goat anti–human ENA-78 (80 ng/mL; R&D Systems) was used as a detecting antibody, and recombinant human ENA-78 (R&D Systems) was used as the standard.

    Western Blot:

    Article Title: Molecular expression of the alpha-chemokine rabbit GRO in Escherichia coli and characterization of its production by lung cells in vitro and in vivo.
    Article Snippet: Polyclonal goat IgG was purified from serum using protein G columns (Pierce), and a portion was biotinylated (Pierce). .. The specificity of the goat antisera was tested by Western blotting using biotinylated goat anti-RabGRO IgG or biotinylated nonimmune IgG and recombinant rabbit IL-8, rabbit monocyte chemotactic protein-1 (MCP-1) (18), recombinant human GRO-a (R & D Systems, Inc., Minneapolis, MN), and rRabGRO protein. .. Radioimmunoprecipitation of Rabbit GRO Protein from Stimulated AM—Rabbit AM (2 3 106 cells/ml) were incubated for 1 h at 37 °C in 24-well plates containing 1 ml of labeling medium containing 250 mCi of [35S]Cys (DuPont NEN).

    Purification:

    Article Title: Chemotactic activity of CXC chemokines interleukin-8, growth-related oncogene-alpha, and epithelial cell-derived neutrophil-activating protein-78 in urine of patients with urosepsis.
    Article Snippet: .. For determination of GRO-a levels, purified monoclonal mouse anti–human GRO-a (4 mg/mL; R&D Systems, Abingdon, UK) was used as a coating antibody, biotinylated affinity-purified goat IgG anti–human GRO-a (20 ng/mL; R&D Systems) was used as a detecting antibody, and recombinant human GRO-a (R&D Systems) was used as the standard. .. For determination of ENA-78, monoclonal mouse anti–human ENA-78 (1 mg/mL; R&D Systems) was used as a coating antibody, biotinylated goat anti–human ENA-78 (80 ng/mL; R&D Systems) was used as a detecting antibody, and recombinant human ENA-78 (R&D Systems) was used as the standard.

    Affinity Purification:

    Article Title: Chemotactic activity of CXC chemokines interleukin-8, growth-related oncogene-alpha, and epithelial cell-derived neutrophil-activating protein-78 in urine of patients with urosepsis.
    Article Snippet: .. For determination of GRO-a levels, purified monoclonal mouse anti–human GRO-a (4 mg/mL; R&D Systems, Abingdon, UK) was used as a coating antibody, biotinylated affinity-purified goat IgG anti–human GRO-a (20 ng/mL; R&D Systems) was used as a detecting antibody, and recombinant human GRO-a (R&D Systems) was used as the standard. .. For determination of ENA-78, monoclonal mouse anti–human ENA-78 (1 mg/mL; R&D Systems) was used as a coating antibody, biotinylated goat anti–human ENA-78 (80 ng/mL; R&D Systems) was used as a detecting antibody, and recombinant human ENA-78 (R&D Systems) was used as the standard.



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